Abundant expression of uncoupling protein-2 in the small intestine: up-regulation by dietary fish oil and fibrates

Citation
T. Murase et al., Abundant expression of uncoupling protein-2 in the small intestine: up-regulation by dietary fish oil and fibrates, BBA-MOL C B, 1530(1), 2001, pp. 15-22
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
ISSN journal
1388-1981 → ACNP
Volume
1530
Issue
1
Year of publication
2001
Pages
15 - 22
Database
ISI
SICI code
1388-1981(20010115)1530:1<15:AEOUPI>2.0.ZU;2-S
Abstract
Mitochondrial uncoupling protein-2 (UCP-2) is widely expressed in various m ammalian tissues, although its physiological functions are not well underst ood. We examined the effects of dietary fish oil on UCP-2 expression in the rat small intestine, in which UCP-2 mRNA levels are higher than in other o rgans. Feeding with fish oil (20%) up-regulated UCP-2 mRNA within 6 days in the small intestine as well as the liver, compared to feeding with soybean oil. This was mimicked by feeding with agonists for peroxisome proliferato r-activated receptor alpha (PPAR alpha) such as fenofibrate and bezafibrate , but not the PPAR gamma agonist troglitazone. The bezafibrate-induced incr ease in UCP-2 expression was found within 2 days in the small intestine, bu t only after 6 days in the liver. The up-regulation of UCP-2 was also found in cultured intestinal epithelial cells (IEC-6) treated for 24 h with vari ous long-chain fatty acids and PPAR alpha agonists. These results indicated that intestinal UCP-2 is up-regulated through direct activation of PPAR al pha by dietary fatty acids. (C) 2001 Elsevier Science B.V. All rights reser ved.