Repression of cyclin B1 expression after treatment with adriamycin, but not cisplatin in human lung cancer A549 cells

Authors
Citation
S. Dan et T. Yamori, Repression of cyclin B1 expression after treatment with adriamycin, but not cisplatin in human lung cancer A549 cells, BIOC BIOP R, 280(3), 2001, pp. 861-867
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006-291X → ACNP
Volume
280
Issue
3
Year of publication
2001
Pages
861 - 867
Database
ISI
SICI code
0006-291X(20010126)280:3<861:ROCBEA>2.0.ZU;2-1
Abstract
Most anticancer drugs cause DNA strand breaks and finally induce cell cycle arrest or cell death. To identify genes involved in these effects, we exam ined gene expression profiles in human lung cancer A549 cells before and af ter adriamycin treatment, using a cDNA array technique. In this manner, we identified several up- or down-regulated genes in cells undergoing G2 arres t following adriamycin treatment; among them, cyclin B1 expression was dram atically reduced. The reduction in cyclin B1 expression and G2 arrest were also seen after treatment with etoposide and irinotecan. Previous reports h ave shown that overexpression of p53 represses cyclin B1 transcription. How ever, cisplatin neither reduced cyclin El expression nor induced G2 phase a rrest, while it induced a comparable amount of p53 protein. These results s uggest that a reduction in cyclin B1 expression plays a role in the mechani sm of action of certain anticancer drugs, including adriamycin, which induc e G2 arrest in cancer cells. (C) 2001 Academic Press.