Virulent strains of Salmonella enteritidis disrupt the epithelial barrier of Caco-2 and HEp-2 cells

Citation
C. Solano et al., Virulent strains of Salmonella enteritidis disrupt the epithelial barrier of Caco-2 and HEp-2 cells, ARCH MICROB, 175(1), 2001, pp. 46-51
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ARCHIVES OF MICROBIOLOGY
ISSN journal
0302-8933 → ACNP
Volume
175
Issue
1
Year of publication
2001
Pages
46 - 51
Database
ISI
SICI code
0302-8933(200101)175:1<46:VSOSED>2.0.ZU;2-4
Abstract
To confirm the existence in nature of Salmonella enteritidis strains of dif ferent degrees of virulence and to elucidate the mechanisms underlying the effects of such strains on the epithelial barrier function, the consequence s of infection of Caco-2 cells and HEp-2 cells with 15 S. enteritidis strai ns in a chicken infection model were examined. The more virulent strains of S. enteritidis, which are biofilm producers in adherence test medium, were able to disrupt HEp-2 and Caco-2 monolayers, as shown by transmonolayer el ectrical resistance and lactate dehydrogenase activity. In contrast, the lo w-virulence strains of S. enteritidis, which do not produce biofilms in adh erence test medium, had no effect on the same cells. An avirulent rough mut ant of Salmonella minnesota exhibited a pattern of behaviour similar to tha t of the low virulence strains of S. enteritidis, whilst a clinical Salmone lla typhi strain caused rapid injury to the monolayers. The effect of super natants of Salmonella cultures in adherence test medium on the integrity of Caco-2 cell monolayers indicated that the high-virulence S. enteritidis st rains, but not the low-virulence strains, release a soluble factor when inc ubated under optimum biofilm-forming conditions, which enables the disrupti on of the integrity of Caco-2 monolayers.