Application of capillary electrophoresis to the simultaneous determinationof betaines in plants

Citation
N. Nishimura et al., Application of capillary electrophoresis to the simultaneous determinationof betaines in plants, ANAL SCI, 17(1), 2001, pp. 103-106
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICAL SCIENCES
ISSN journal
0910-6340 → ACNP
Volume
17
Issue
1
Year of publication
2001
Pages
103 - 106
Database
ISI
SICI code
0910-6340(200101)17:1<103:AOCETT>2.0.ZU;2-P
Abstract
The simultaneous determination of betaines, the key compounds for osmotic r egulation in plants, was established by capillary electrophoresis (CE). Aft er four betaines, glycine betaine (GB), beta -alanine betaine (AB), proline betaine (PB), and 2-hydroxyproline betaine (HPB), were esterified with p-b romophenacyl bromide, the esters were electrophoresed in 100 mM sodium phos phate at pH 3.0. A low-pH condition in CE and esterification gave an advant age of resolving each of the ester peaks as well as those of the unreacted reagent and other components. Furthermore, the addition of 4% polyethylene glycol (PEG) gave a better resolution of 4 peaks, resulting in the separati on of the overlapped peaks of PB and AB. It was found from the standard add ition method being applied to barley leaves that the GB content in plants c ould be evaluated by using a calibration curve of the GB standard solution. The extraction of GB from plant leaves was also improved by adopting water as the extraction solvent instead of a mixture of organic solvents. The pr esent method was suitably applied to actual plant specimens collected from a saline area of China.