Integrin pattern and effect on contraction in cultured testicular peritubular myoid cells

Citation
M. Magnanti et al., Integrin pattern and effect on contraction in cultured testicular peritubular myoid cells, AM J REPROD, 45(1), 2001, pp. 21-27
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
ISSN journal
1046-7408 → ACNP
Volume
45
Issue
1
Year of publication
2001
Pages
21 - 27
Database
ISI
SICI code
1046-7408(200101)45:1<21:IPAEOC>2.0.ZU;2-T
Abstract
PROBLEM: Neither the integrin pattern nor the biological functions of integ rins have been extensively documented in human cultured testicular peritubu lar myoid cells (TPMC). The integrin pattern and the presence of some prote ins of the immunoglobulin superfamily on human TPMC as well as the role of integrins in TPMC contraction were examined. METHOD OF STUDY: Integrin expression was evaluated bq immunofluorescence an d FACS analysis. To assess the role of integrin in TPMC contraction, human and rat cells were added to a collagen gel system and exposed to contractil e stimuli. RESULTS: The immunofluorescence and cytofluorimetric analysis showed that h uman cultured TPMC express alpha (1), alpha (2), alpha (3), alpha (5), alph a (6), alpha (v), beta (1), beta (3), and beta (4) integrin subunits, and s ignificant amounts of intercellular adhesion molecule-1 (ICAM-1), whereas t hey do not present alpha (4), beta (2), beta (7) subunits, nor intercellula r adhesion molecule-2 (ICAM-2) and neural cell adhesion molecule (NCAM). Th e preincubation of human cells with an anti-beta (1) mAb and of rat cells w ith a polyclonal anti-beta (1) antibody inhibited TPMC contraction induced by different contractile stimuli. CONCLUSION: Our investigation documented a broad integrin pattern on human cultured TPMC as well as a role for integrins in human and rat TPMC contrac tion.