Effects of Mycobacterium bovis BCG on the development of allergic inflammation and bronchial hyperresponsiveness in hyper-IgE BP2 mice vaccinated as newborns

Citation
Ma. Nahori et al., Effects of Mycobacterium bovis BCG on the development of allergic inflammation and bronchial hyperresponsiveness in hyper-IgE BP2 mice vaccinated as newborns, VACCINE, 19(11-12), 2001, pp. 1484-1495
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Veterinary Medicine/Animal Health",Immunology
Journal title
VACCINE
ISSN journal
0264-410X → ACNP
Volume
19
Issue
11-12
Year of publication
2001
Pages
1484 - 1495
Database
ISI
SICI code
0264-410X(20010108)19:11-12<1484:EOMBBO>2.0.ZU;2-T
Abstract
Asthma may result from excessive Th-2 response in children not previously e xposed to Th-l-inducing infections. We tested the hypothesis that BCG vacci nation in Th-2-susceptible newborn BP2 mice blocks allergic inflammation an d bronchial hyperreactivity (BHR). Ten day-old BP2 mice received 10(5) CFU of BCG 1173P2 intranasally (IN), and 6, 10 or 14 weeks thereafter were sens itized with 100 mug ovalbumin (OVA) in aluminium hydroxide twice subcutaneo usly (SC) at 1 week interval, and challenged 1 week after the second sensit ization with 10 mug OVA IN. Compared to OVA-challenged unvaccinated mice, t hose that received BCG 8 weeks before challenge developed intense bronchial inflammation, BHR, and high IgE titers. Inflammation involved T cells, mac rophages, dendritic cells and was accompanied by increased levels of Interl eukin-5 (IL-5) in the bronchoalveolar lavages (BAL). However, animals chall enged 16 weeks after BCG vaccination did not develop BHR nor bronchial hype reosinophilia, and showed reduced IgE levels. Bronchial infiltration by imm unocompetent cells was also significantly reduced. Increased levels of gamm a-interferon (IFN-gamma) after in vitro stimulation of tracheo-bronchial ly mph node cells accompanied this blockage, but levels of IL-5 remained high. We demonstrate that 16 weeks after vaccination with BCG in newborn BP2 mic e which have a high Th-2 background, allergic inflammation and BHR were blo cked, even though a clear Th-l shift was not achieved. (C) 2001 Elsevier Sc ience Ltd. All rights reserved.