Mucosal immunization against hepatitis B virus by intranasal co-administration of recombinant hepatitis B surface antigen and recombinant cholera toxin B subunit as an adjuvant

Citation
M. Isaka et al., Mucosal immunization against hepatitis B virus by intranasal co-administration of recombinant hepatitis B surface antigen and recombinant cholera toxin B subunit as an adjuvant, VACCINE, 19(11-12), 2001, pp. 1460-1466
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Veterinary Medicine/Animal Health",Immunology
Journal title
VACCINE
ISSN journal
0264-410X → ACNP
Volume
19
Issue
11-12
Year of publication
2001
Pages
1460 - 1466
Database
ISI
SICI code
0264-410X(20010108)19:11-12<1460:MIAHBV>2.0.ZU;2-M
Abstract
Recombinant cholera toxin B subunit (rCTB) produced by Bacillus brevis carr ying pNU212-CTB has been previously found to be a potent mucosal adjuvant t o aluminium-non-adsorbed tetanus toroid (nTT) and diphtheria toroid (nDT) c o-administered intranasally, and the possibility of needle-free inoculation of these vaccines with rCTB has been suggested. In this paper we examined the potentiality of rCTB as a mucosal adjuvant to aluminium-non-adsorbed ye ast-derived recombinant hepatitis B surface antigen (rHBs) being a particul ate antigen when administered intranasally with rCTB. In-house ELISA showed that a mixture of rHBs (1 or 5 mug) and rCTB (10 mug) elevated not only sy stemic responses but also mucosal immune responses at the nasal cavity. the lung, the saliva, the small intestine and the vagina against rHBs, and the se could be further increased with higher doses of antigen. With antibody i sotypes of IgG, there were equally high levels of serum HBs-specific IgG1, IgG2a and IgG2b antibodies and induction of mixed Th1 and Th2-type response s was considered to occur in combination of rHBs and rCTB. Serum anti-HBs t itres in almost all mice obtained from sandwich EIA using a commercial kit were higher than 1000 milli-international units ml (-1) (mIU ml(-1)). These results show that rCTB is also very effective as a mucosal adjuvant for a particulate antigen like rHBs, as well as soluble antigens like nTT and nDT reported previously, suggesting the possibility of intranasal immunization with rHBs plus rCTB in humans. (C) 2001 Elsevier Science Ltd. All rights r eserved.