Metabolism of the herbicide glufosinate-ammonium in plant cell cultures oftransgenic (rhizomania-resistant) and non-transgenic sugarbeet (Beta vulgaris), carrot (Daucus carota), purple foxglove (Digitalis purpurea) and thorn apple (Datura stramonium)

Citation
Bp. Muller et al., Metabolism of the herbicide glufosinate-ammonium in plant cell cultures oftransgenic (rhizomania-resistant) and non-transgenic sugarbeet (Beta vulgaris), carrot (Daucus carota), purple foxglove (Digitalis purpurea) and thorn apple (Datura stramonium), PEST MAN SC, 57(1), 2001, pp. 46-56
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Entomology/Pest Control
Journal title
PEST MANAGEMENT SCIENCE
ISSN journal
1526-498X → ACNP
Volume
57
Issue
1
Year of publication
2001
Pages
46 - 56
Database
ISI
SICI code
1526-498X(200101)57:1<46:MOTHGI>2.0.ZU;2-D
Abstract
The metabolism of the herbicide glufosinate-ammonium was investigated in he terotrophic cell suspension and callus cultures of transgenic (bar-gene) an d non-transgenic sugarbeet (Beta vulgaris). Similar studies were performed with suspensions of carrot (Daucus carota), purple foxglove (Digitalis purp urea) and thorn apple (Datura stramonium). C-14-labelled chemicals were the (racemic) glufosinate, L-glufosinate, and D-glufosinate, as well as the me tabolites N-acetyl L-glufosinate and 3-(hydroxymethylphosphinyl)propionic a cid (MPP). Cellular absorption was generally low, but depended noticeably o n plant species, substance and enantiomer. Portions of non-extractable resi dues ranged from 0.1% to 1.2% of applied C-14. Amounts of soluble metabolit es resulting from glufosinate or L-glufosinate were between 0.0% and 26.7% of absorbed C-14 in non-transgenic cultures and 28.2% and 59.9% in transgen ic sugarbeet. D-Glufosinate, MPP and N-acetyl L-glufosinate proved to be st able. The main metabolite in transgenic sugarbeet was N-acetyl L-glufosinat e, besides traces of MPP and 4-(hydroxymethylphosphinyl)butanoic acid (MPB) . In non-transgenic sugarbeet, glufosinate was transformed to a limited ext ent to MPP and trace amounts of MPB. In carrot, D stramonium and D purpurea , MPP was also the main product; MPB was identified as a further trace meta bolite in D stramonium and D purpurea. (C) 2001 Society of Chemical Industr y.