Rapid isolation of tissue-specific genes from rat kidney

Hu, ED Chen, ZX Fredrickson, TA Spurr, N Gentle, S Sims, M Zhu, Y Halsey, W Van Horn, S Mao, J Sathe, GM Brooks, DP

Ed. Hu et al., Rapid isolation of tissue-specific genes from rat kidney, EXP NEPHROL, 9(2), 2001, pp. 156-164

32

INGLESE

Article

Urology & Nephrology","da verificare

EXPERIMENTAL NEPHROLOGY

1018-7782 → ACNP

9

2

2001

156 - 164

ISI

1018-7782(200103/04)9:2<156:RIOTGF>2.0.ZU;2-H

A systematic effort to isolate kidney-specific genes was performed using re cently described PCR-select methodology. Using this technique, a kidney-spe cific mini-gene library was generated and a number of kidney-specific genes that share significant homology to previously characterized kidney genes f rom rats and other species were isolated. These included three renal-specif ic transporters (an ADH water channel, the anion transporters RST and ROAT1 ), a cell adhesion molecule (K-cadherin) and a kidney-specific protein upre gulated in renal carcinoma (DD96). In addition, we isolated two novel genes from a rat kidney. One of the genes shares limited homology to rat profili n-1 while the other did not share any similarity to genes in the Genbank. N orthern blot analysis revealed that the mRNA for each of these genes is exp ressed in a highly kidney-restricted fashion. Our results suggested that ti ssue-specific genes can be rapidly isolated and characterized using PCR-sel ect techniques and this methodology may be generally applicable to isolate specific genes from a variety of tissues. Copyright (C) 2001 S. Karger AG, Basel.