H. Funabashi et al., Establishment of a Tcrb and Trp53 genes deficient mouse strain as an animal model for spontaneous colorectal cancer, EXP ANIM, 50(1), 2001, pp. 41-47
A congenic C57BL/6JJcl-Tcrb(tm1Mom)Trp53(tm1) (Tcrb(-/-): Trp53(-/-)) mouse
lacking T-cell receptor beta chain (TCR beta) and transformation related p
rotein 53 (p53) has been established at the N8th generation of backcrossing
male Tcrb(-/-):Trp53(-/-) mice, which had been obtained by mating a Tcrb(-
/-) mouse with a Trp53(-/-) mouse, with female C57BL/6JJcl mice, In the mic
e deficient for the both genes, occurrence of tumor masses was observed mos
tly in the cecum with high frequency as examined at 3 months of age. The ma
jority of the masses had histologic features of hyperplasia or dysplasia wh
ile occasional lesions were noted to be adenocarcinomas invading the submuc
osa (invasive adenocarcinoma). As examined at 4 months of age and thereafte
r, ail mice had 4-5 colorectal tumors per animal, the lesions being located
mainly in the cecum and, histopathologically, all the obvious neoplastic g
rowths in the regions examined were invasive adenocarcinomas. The Tcrb and
Trp53 genes deficient mouse strain which develops spontaneous colorectal ca
rcinoma with fairly high frequency at early age would be useful as an anima
l model for colorectal cancer.