Establishment of a Tcrb and Trp53 genes deficient mouse strain as an animal model for spontaneous colorectal cancer

Citation
H. Funabashi et al., Establishment of a Tcrb and Trp53 genes deficient mouse strain as an animal model for spontaneous colorectal cancer, EXP ANIM, 50(1), 2001, pp. 41-47
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Animal Sciences
Journal title
EXPERIMENTAL ANIMALS
ISSN journal
1341-1357 → ACNP
Volume
50
Issue
1
Year of publication
2001
Pages
41 - 47
Database
ISI
SICI code
1341-1357(200101)50:1<41:EOATAT>2.0.ZU;2-W
Abstract
A congenic C57BL/6JJcl-Tcrb(tm1Mom)Trp53(tm1) (Tcrb(-/-): Trp53(-/-)) mouse lacking T-cell receptor beta chain (TCR beta) and transformation related p rotein 53 (p53) has been established at the N8th generation of backcrossing male Tcrb(-/-):Trp53(-/-) mice, which had been obtained by mating a Tcrb(- /-) mouse with a Trp53(-/-) mouse, with female C57BL/6JJcl mice, In the mic e deficient for the both genes, occurrence of tumor masses was observed mos tly in the cecum with high frequency as examined at 3 months of age. The ma jority of the masses had histologic features of hyperplasia or dysplasia wh ile occasional lesions were noted to be adenocarcinomas invading the submuc osa (invasive adenocarcinoma). As examined at 4 months of age and thereafte r, ail mice had 4-5 colorectal tumors per animal, the lesions being located mainly in the cecum and, histopathologically, all the obvious neoplastic g rowths in the regions examined were invasive adenocarcinomas. The Tcrb and Trp53 genes deficient mouse strain which develops spontaneous colorectal ca rcinoma with fairly high frequency at early age would be useful as an anima l model for colorectal cancer.