A. Clad et al., Detection of seroconversion and persistence of Chlamydia trachomatis antibodies in five different serological tests, EUR J CL M, 19(12), 2000, pp. 932-937
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
Microimmunofluorescence (MIF), a Chlamydia trachomatis species-specific enz
yme immunoassay incorporating lipopolysaccharide-extracted Chlamydia tracho
matis L2 elementary bodies, two different synthetic peptide-based species-s
pecific tests, and a recombinant lipopolysaccharide genus-specific test wer
e performed on multiple follow-up sera (n = 104 total) from 16 women with C
hlamydia trachomatis-positive cervical swabs. These women included five wit
h IgG seroconversions, five with Chlamydia trachomatis reinfections after i
nitial therapy, and six with serologic follow-up of more than 6 years after
antibiotic therapy. Of all the tests employed in this study, MIF IgG rever
ted earliest to negative titers, while MIF IgA was the least sensitive. The
lipopolysaccharide-extracted elementary body enzyme immunoassay exhibited
the closest correlation with the MIF test. The highest test sensitivity was
observed in one of the synthetic peptide-based tests, which detected earli
est seroconversions and longest IgG persistence. The other synthetic peptid
e-based test gave false-negative results in 2 of 16 women and did not detec
t seroconversion earlier than the MIF test. Seroconversion and persistence
of genus-specific IgG - cross-reactivity with Chlamydia pneumoniae - agains
t lipopolysaccharide were similar to species-specific IgG. A significant se
rologic response to reinfection was observed only in women with signs of pe
lvic inflammatory disease. Species-specific tests of high sensitivity and r
eproducibility are best suited for gynecological diagnostic purposes.