Detection of seroconversion and persistence of Chlamydia trachomatis antibodies in five different serological tests

Citation
A. Clad et al., Detection of seroconversion and persistence of Chlamydia trachomatis antibodies in five different serological tests, EUR J CL M, 19(12), 2000, pp. 932-937
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
ISSN journal
0934-9723 → ACNP
Volume
19
Issue
12
Year of publication
2000
Pages
932 - 937
Database
ISI
SICI code
0934-9723(200012)19:12<932:DOSAPO>2.0.ZU;2-V
Abstract
Microimmunofluorescence (MIF), a Chlamydia trachomatis species-specific enz yme immunoassay incorporating lipopolysaccharide-extracted Chlamydia tracho matis L2 elementary bodies, two different synthetic peptide-based species-s pecific tests, and a recombinant lipopolysaccharide genus-specific test wer e performed on multiple follow-up sera (n = 104 total) from 16 women with C hlamydia trachomatis-positive cervical swabs. These women included five wit h IgG seroconversions, five with Chlamydia trachomatis reinfections after i nitial therapy, and six with serologic follow-up of more than 6 years after antibiotic therapy. Of all the tests employed in this study, MIF IgG rever ted earliest to negative titers, while MIF IgA was the least sensitive. The lipopolysaccharide-extracted elementary body enzyme immunoassay exhibited the closest correlation with the MIF test. The highest test sensitivity was observed in one of the synthetic peptide-based tests, which detected earli est seroconversions and longest IgG persistence. The other synthetic peptid e-based test gave false-negative results in 2 of 16 women and did not detec t seroconversion earlier than the MIF test. Seroconversion and persistence of genus-specific IgG - cross-reactivity with Chlamydia pneumoniae - agains t lipopolysaccharide were similar to species-specific IgG. A significant se rologic response to reinfection was observed only in women with signs of pe lvic inflammatory disease. Species-specific tests of high sensitivity and r eproducibility are best suited for gynecological diagnostic purposes.