Selective transmission of RS-tropic HIV type 1 from dendritic cells to resting CD4(+) T cells

Citation
Sa. David et al., Selective transmission of RS-tropic HIV type 1 from dendritic cells to resting CD4(+) T cells, AIDS RES H, 17(1), 2001, pp. 59-68
Citations number
65
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
AIDS RESEARCH AND HUMAN RETROVIRUSES
ISSN journal
0889-2229 → ACNP
Volume
17
Issue
1
Year of publication
2001
Pages
59 - 68
Database
ISI
SICI code
0889-2229(20010101)17:1<59:STORHT>2.0.ZU;2-A
Abstract
In an in vitro coculture model of monocyte-derived, cultured human dendriti c cells (DC) with autologous CD4(+) resting T cells, CCR5 (RS)-tropic strai ns of HIV-1, but not CXCR4 (X4)-tropic strains, were transmitted to resting CD4(+) T cells, leading to prolific viral output, although DC were suscept ible to infection with either strain. Macrophages, which were also infectab le with either R5- or X4-tropic strains, did not transmit infection to CD4( +) cells. Highly productive HIV infection in this model appeared to be a co nsequence of heterokaryotic syncytium formation between infected DC and T c ells since syncytia formation developed only in R5-infected DC/CD4(+) cocul tures. These results suggested that the unique microenvironment derived fro m the fusion between the infected DC and CD4(+) cell was highly permissive and selective for replication of R5-tropic viruses. The apparent selectivit y for R5-tropic strains in such syncytia was attributable neither to differ ential DC-mediated activation nor to selective modulation of induction of a lpha- or beta -chemokines in the infected DC. This model of HIV replication may provide useful insights into in vitro correlates of HIV pathogenicity.