Identification of many crystal forms of Aspergillus nidulans dehydroquinate synthase

Citation
Ce. Nichols et al., Identification of many crystal forms of Aspergillus nidulans dehydroquinate synthase, ACT CRYST D, 57, 2001, pp. 306-309
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
0907-4449 → ACNP
Volume
57
Year of publication
2001
Part
2
Pages
306 - 309
Database
ISI
SICI code
0907-4449(200102)57:<306:IOMCFO>2.0.ZU;2-J
Abstract
Extensive crystallization trials of Aspergillus nidulans dehydroquinate syn thase, a potential novel target for antimicrobial drugs, in complexes with different ligands have resulted in the identification of nine crystal forms . Crystals of unliganded DHQS, binary complexes with either the substrate a nalogue, carbaphosphonate or the cofactor NADH, as well as the ternary DHQS -carbaphosphonate-cofactor complex, were obtained. The ternary complex crys tallizes from ammonium sulfate and CoCl2 in space group P2(1)2(1)2, with un it-cell parameters a = 133.8, b = 86.6, c = 74.9 Angstrom. The binary carba phosphonate complex crystallizes from PEG 6000 in space group P2(1)2(1)2(1) , with a = 70.0, b = 64.0, c = 197.6 Angstrom, and the binary cofactor comp lex crystallizes from PEG 3350 and sodium potassium tartrate in space group P2(1), with a = 83.7, b = 70.4, c = 144.3 Angstrom, beta = 89.2 degrees. D HQS in the absence of ligands crystallizes in space group P2(1), with a = 4 1.0, b = 68.9, c = 137.7 Angstrom, beta = 94.8 degrees. Each of these cryst al forms are suitable for high-resolution structure determination. Structur es of a range of DHQS-ligand complexes will be of value in the structure-ba sed design of novel antimicrobial drugs.