Quantitative analysis model for fiber-optic chemical sensors based on fluorescence quenching for analytes and its application

Citation
W. Li et al., Quantitative analysis model for fiber-optic chemical sensors based on fluorescence quenching for analytes and its application, ACT CHIM S, 59(1), 2001, pp. 109-114
Citations number
9
Language
CHINESE
art.tipo
Article
Categorie Soggetti
Chemistry
Journal title
ACTA CHIMICA SINICA
ISSN journal
0567-7351 → ACNP
Volume
59
Issue
1
Year of publication
2001
Pages
109 - 114
Database
ISI
SICI code
0567-7351(2001)59:1<109:QAMFFC>2.0.ZU;2-S
Abstract
Because the fiber - optic chemical sensors based on the fluorescence quench ing has an adjustable short distance from the membrane to the end of fiber optics, forming a space of microcell, a diminution of fluorescence is induc ed by inner filter effects, involving absorption of both excited light from the light source and emitted light from the membrane. If the absorption sp ectra of analytes overlap the fluorescence excitation and/or emission spect ra of the fluoroprobe in the membrane, the quenching signal of the fiber - optic chemical sensors would be produced by the resonance energy transfer. In addition, dynamic quenching happens to some haloid, heavy metal compound s and aromatic nitro organic compounds. According to the structural charact erization of the filer - optic chemical sensors based on fluorescence quenc hing and the mechanism of fluorescence multiple quenching, a non - linear m athematical model was. deduced and described for the quantitative analysis model for the fiber - optic chemical sensors. A multiple model regression t echnique for the quantitative analysis model was reported to provide rapidl y and directly the relations between the response signal and the concentrat ion of analytes and establish linear regression equation for predicting the concentration of analytes. The technique was applied to fit best mathemati cal model from the control samples of the therapeutic drugs based on the re sponse of fiber - optic chemical sensors, such as metronidazole, nitrofuran toin and ofloxacin. The pyrenebutyric acid was chosen as a fluoroprobe for constructing the filer - optic chemical sensor to response the samples. The experimental results showed that these models had some good characteristic s and gave an alternative method for establishing quantitative analysis mod els for the filer - optic chemical sensors.