Apoptosis and mitosis of parenchymal cells in the duct-ligated rat submandibular gland

Citation
S. Takahashi et al., Apoptosis and mitosis of parenchymal cells in the duct-ligated rat submandibular gland, TISSUE CELL, 32(6), 2000, pp. 457-463
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
TISSUE & CELL
ISSN journal
0040-8166 → ACNP
Volume
32
Issue
6
Year of publication
2000
Pages
457 - 463
Database
ISI
SICI code
0040-8166(200012)32:6<457:AAMOPC>2.0.ZU;2-O
Abstract
Apoptosis and proliferation of parenchymal cells during atrophy of rat subm andibular gland induced by double duct ligation were investigated using imm unohistochemistry for proliferating cell nuclear antigen (PCNA), terminal d eoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick end labell ing (TUNEL) and transmission electron microscopy (TEM). At 2 and 3 days aft er ligation, increased PCNA positive cells and mitoses were seen in ducts; thereafter PCNA positive cells decreased in number. At 3 and 4 days, the ac inar cell population rapidly decreased, with many remaining TUNEL positive acinar cells. During this period, TEM showed typical apoptotic acinar cells that were phagocytosed by adjacent acinar cells or intraepithelial macroph ages, After 7 days, most acinar cells had disappeared, leaving prominent re sidual ducts; a few acinar cells remained, especially at the lobule periphe ry. Submandibular gland duct ligation thus induced marked depletion of acin ar cell by apoptosis and a concurrent short-lived cycle of duct cell prolif eration. (C) 2000 Harcourt Publishers Ltd.