Additive effect of mPer1 and mPer2 antisense oligonucleotides on light-induced phase shift

Citation
H. Wakamatsu et al., Additive effect of mPer1 and mPer2 antisense oligonucleotides on light-induced phase shift, NEUROREPORT, 12(1), 2001, pp. 127-131
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROREPORT
ISSN journal
0959-4965 → ACNP
Volume
12
Issue
1
Year of publication
2001
Pages
127 - 131
Database
ISI
SICI code
0959-4965(20010122)12:1<127:AEOMAM>2.0.ZU;2-4
Abstract
It is well known that light induces both mPer1 and mPer2 mRNA in the suprac hiasmatic nucleus. We have reported that mPer1 antisense oligonucleotides ( ODNs) inhibited the light-induced phase delays of mouse locomotor rhythm. I n this study, we asked whether both or either mPer1 or mPer2 expression is necessary to induce the phase shift. We examined the effects of inhibition of mRNA expression on light-induced phase delays of mouse circadian behavio r rhythm. Light-induced phase delays were moderately attenuated by microinj ection of mPer1 or mPer2 antisense ODN, but not by mPer3 antisense or mPer1 , mPer2 scrambled ODNs, whereas following simultaneous injection of both mP er1 and mPer2 antisense ODNs they disappeared. The present results suggest that acute induction of mPer1 and mPer2 gene play an additive effect on pho tic entrainment. NeuroReport 12:127-131 (C) 2001 Lippincott Williams & Wilk ins.