Increased levels of oxidative stress markers detected in the brains of mice devoid of prion protein

Citation
Bs. Wong et al., Increased levels of oxidative stress markers detected in the brains of mice devoid of prion protein, J NEUROCHEM, 76(2), 2001, pp. 565-572
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
JOURNAL OF NEUROCHEMISTRY
ISSN journal
0022-3042 → ACNP
Volume
76
Issue
2
Year of publication
2001
Pages
565 - 572
Database
ISI
SICI code
0022-3042(200101)76:2<565:ILOOSM>2.0.ZU;2-V
Abstract
Although minor abnormalities have been reported in prion protein (PrP) knoc k-out (Prnp(-/-)) mice, the normal physiological function of PrP, the causa tive agent implicated in transmissible spongiform encephalopathies (TSE), r emains unresolved. Since there are increasing correlations between oxidativ e stress and amyloidoses, we decided to investigate whether PrP plays a rol e in oxidative modulation. We found higher levels of oxidative damage to pr oteins and lipids in the brain lysates of Prnp(-/-) as compared to wild-typ e (WT) mice of the same genetic background. These two indicators, protein o xidation and lipid peroxidation, are hallmarks of cellular oxidative damage . Elevated levels of ubiquitin-protein conjugates were also observed in Prn p(-/-) mice, a probable consequence of cellular attempts to remove the dama ged proteins as indicated by increased proteasome activity. Taken together, these findings are indicative of a role for PrP in oxidative homeostasis i n vivo.