Localization of the protein L2 in the 50 S subunit and the 70 S E-coli ribosome

Citation
R. Willumeit et al., Localization of the protein L2 in the 50 S subunit and the 70 S E-coli ribosome, J MOL BIOL, 305(1), 2001, pp. 167-177
Citations number
49
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
JOURNAL OF MOLECULAR BIOLOGY
ISSN journal
0022-2836 → ACNP
Volume
305
Issue
1
Year of publication
2001
Pages
167 - 177
Database
ISI
SICI code
0022-2836(20010105)305:1<167:LOTPLI>2.0.ZU;2-P
Abstract
The protein L2 is found in all ribosomes and is one of the best conserved p roteins of this mega-dalton complex. The protein was localized within both the isolated 50 S subunit and the 70 S ribosome of the Escherichia coli bac teria with the neutron-scattering technique of spin-contrast variation. L2 is elongated, exposing one end of the protein to the surface of the intersu bunit interface of the 50 S subunit. The protein changes its conformation s lightly when the 50 S subunit reassociates with the 30 S subunit to form a 70 S ribosome, becoming more elongated and moving approximately 30 ii into the 50 S matrix. The results support a recent observation that L2 is essent ial for the association of the ribosomal subunits and might participate in the binding and translocation of the tRNAs. (C) 2001 Academic Press.