Effect of liquid chromatography separation of complex matrices on liquid chromatography-tandem mass spectrometry signal suppression

Citation
Bk. Choi et al., Effect of liquid chromatography separation of complex matrices on liquid chromatography-tandem mass spectrometry signal suppression, J CHROMAT A, 907(1-2), 2001, pp. 337-342
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A
ISSN journal
0021-9673 → ACNP
Volume
907
Issue
1-2
Year of publication
2001
Pages
337 - 342
Database
ISI
SICI code
0021-9673(20010112)907:1-2<337:EOLCSO>2.0.ZU;2-M
Abstract
The effect of liquid chromatography separation on liquid chromatogaphy-tand em mass spectrometry (LC-MS-MS) signal response for the characterization of low-molecular-mass compounds in a complex matrix was investigated. Matrix induced signal suppression appears throughout the entire LC-MS-MS analysis of wheat forage extract, with greatest suppression occurring at early reten tion times. Experimental results show that co-elution of matrix components and analytes from the LC column may be most strongly attributed to column o verloading rather than similar analyte and matrix retention behavior. As a result, two-dimensional (LC-LC) separation can be a highly effective approa ch to address signal suppression effects for the quantitative LC-MS-MS anal ysis of complex matrix samples. (C) 2001 Elsevier Science B.V. All rights r eserved.