The 70-kDa major DNA-compacting protein of the chloroplast nucleoid is sulfite reductase

Citation
N. Sato et al., The 70-kDa major DNA-compacting protein of the chloroplast nucleoid is sulfite reductase, FEBS LETTER, 487(3), 2001, pp. 347-350
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
0014-5793 → ACNP
Volume
487
Issue
3
Year of publication
2001
Pages
347 - 350
Database
ISI
SICI code
0014-5793(20010105)487:3<347:T7MDPO>2.0.ZU;2-V
Abstract
The chloroplast nucleoid is a complex of chloroplast DNA and various, mostl y uncharacterized proteins. An abundant 70-kDa protein of the isolated nucl eoids of pea chloroplasts was identified as sulfite reductase by N-terminal sequence analysis as well as immunoblot analysis, spectrophotometry and en zyme activity analysis. Recombinant maize sulfite reductase was indeed able to compact chloroplast DNA and to form nucleoid-like particles in vitro. T he role of sulfite reductase in the structural organization of the nucleoid is discussed. (C) 2001 Federation of European Biochemical Societies. Publi shed by Elsevier Science B.V. All rights reserved.