S. Fukuda et al., Murine and human SDF2L1 is an endoplasmic reticulum stress-inducible gene and encodes a new member of the Pmt/rt protein family, BIOC BIOP R, 280(1), 2001, pp. 407-414
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
We isolated murine and human cDNAs for SDF2L1 (Stromal cell-derived factor
2-like1) and characterized the genomic structures. Northern blot analysis o
f the gene expression in various tissues revealed that both murine Sdf2l1 a
nd human SDF2L1 genes are expressed ubiquitously with particularly high exp
ression in the testis, The SDF2L1 protein has an endoplasmic reticulum (ER)
-retention-like motif, HDEL, at the carboxy (C)-terminus. Interestingly, SD
F2L1 protein also shows significant similarity to the central hydrophilic p
art of protein O-mannosyltransferase (Pmt) proteins of Saccharomyces cerevi
siae, the human homologues of Pmt (POMT1 and POMT2) and Drosophila melanoga
ster rotated abdomen (rt) protein. In a murine hepatocellular carcinoma cel
l line, Sdf2l1 was strongly induced by tunicamycin and a calcium ionophore,
A23187, and weakly induced by heat stress but was not induced by cyclohexi
mide, In conclusion, SDF2L1 protein is a new member of Pmt/rt protein famil
y and Sdf2l1 is a new ER stress-inducible gene, (C) 2001 Academic Press.