Murine and human SDF2L1 is an endoplasmic reticulum stress-inducible gene and encodes a new member of the Pmt/rt protein family

Citation
S. Fukuda et al., Murine and human SDF2L1 is an endoplasmic reticulum stress-inducible gene and encodes a new member of the Pmt/rt protein family, BIOC BIOP R, 280(1), 2001, pp. 407-414
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006-291X → ACNP
Volume
280
Issue
1
Year of publication
2001
Pages
407 - 414
Database
ISI
SICI code
0006-291X(20010112)280:1<407:MAHSIA>2.0.ZU;2-D
Abstract
We isolated murine and human cDNAs for SDF2L1 (Stromal cell-derived factor 2-like1) and characterized the genomic structures. Northern blot analysis o f the gene expression in various tissues revealed that both murine Sdf2l1 a nd human SDF2L1 genes are expressed ubiquitously with particularly high exp ression in the testis, The SDF2L1 protein has an endoplasmic reticulum (ER) -retention-like motif, HDEL, at the carboxy (C)-terminus. Interestingly, SD F2L1 protein also shows significant similarity to the central hydrophilic p art of protein O-mannosyltransferase (Pmt) proteins of Saccharomyces cerevi siae, the human homologues of Pmt (POMT1 and POMT2) and Drosophila melanoga ster rotated abdomen (rt) protein. In a murine hepatocellular carcinoma cel l line, Sdf2l1 was strongly induced by tunicamycin and a calcium ionophore, A23187, and weakly induced by heat stress but was not induced by cyclohexi mide, In conclusion, SDF2L1 protein is a new member of Pmt/rt protein famil y and Sdf2l1 is a new ER stress-inducible gene, (C) 2001 Academic Press.