Perfusion with lipopolysaccharide negative blood eliminates lipopolysaccharide induced lung injury

S. Sasaki et al., Perfusion with lipopolysaccharide negative blood eliminates lipopolysaccharide induced lung injury, ASAIO J, 47(1), 2001, pp. 45-49
Citations number
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology
Journal title
ISSN journal
1058-2916 → ACNP
Year of publication
45 - 49
SICI code
We investigated whether perfusion with control blood improves pulmonary fun ctions compromised by lipopolysaccharide (LPS) infusion. This was an animal study in a research laboratory at a university hospital by using Sprague-D awley rats (n = 19), each weighing 325 to 350 g. All animals were pretreate d with a 24 hour infusion of either LPS (5 mg/kg) or vehicle, after which, excised lungs were reperfused for 2 hours with either LPS+ or control blood . Three groups were studied: (1) group S (n = 6); LPS pretreated lungs repe rfused with LPS containing blood to mimic persistent sepsis, (2) group N (n = 6); LPS pretreated lungs reperfused with control blood to mimic the remo val of the septic blood components, and (3) group C (n = 7); vehicle pretre ated lungs reperfused with normal blood as a control. Blood gas exchange, s hunt fraction (Qs/Qt), alveolar-arterial oxygen gradient (A-aDO(2),), and v ariables for lung mechanics were measured. Leukosequestration was quantifie d with a myeloperoxidase (MPO) assay. The pO(2) (mm Hg) values at 90 min af ter reperfusion in groups S, N, and C were 67.8 +/- 7.0*, 85.2 +/- 9.2, and 90.1 +/- 7.5, respectively (*p < 0.05; vs. group N and C). In addition to pO(2), A-aDO(2) and Qs/Qt significantly deteriorated in group S. MPO activi ty in the lungs after LPS infusion was significantly higher than that after vehicle infusion (1.7 +/- 0.3 vs. 0.12 +/- 0.04 units/g tissue; p < 0.001) . Subsequent reperfusion with LPS+ blood (group S) increased MPO activity t o 3.1 +/- 0.6 (p < 0.05), but reperfusion with normal blood (group N) cause d a significant decrease to 1.1 +/- 0.2 (p < 0.05). MPO activity in group C did not significantly change compared with those after vehicle infusion. R eperfusion with control blood normalized lung function compromised by pretr eatment with LPS and significantly reduced leukosequestration. These result s favor the possibility that the removal of LPS+ blood components may elimi nate septic lung injury.