Perfusion with lipopolysaccharide negative blood eliminates lipopolysaccharide induced lung injury

Citation
S. Sasaki et al., Perfusion with lipopolysaccharide negative blood eliminates lipopolysaccharide induced lung injury, ASAIO J, 47(1), 2001, pp. 45-49
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology
Journal title
ASAIO JOURNAL
ISSN journal
1058-2916 → ACNP
Volume
47
Issue
1
Year of publication
2001
Pages
45 - 49
Database
ISI
SICI code
1058-2916(200101/02)47:1<45:PWLNBE>2.0.ZU;2-O
Abstract
We investigated whether perfusion with control blood improves pulmonary fun ctions compromised by lipopolysaccharide (LPS) infusion. This was an animal study in a research laboratory at a university hospital by using Sprague-D awley rats (n = 19), each weighing 325 to 350 g. All animals were pretreate d with a 24 hour infusion of either LPS (5 mg/kg) or vehicle, after which, excised lungs were reperfused for 2 hours with either LPS+ or control blood . Three groups were studied: (1) group S (n = 6); LPS pretreated lungs repe rfused with LPS containing blood to mimic persistent sepsis, (2) group N (n = 6); LPS pretreated lungs reperfused with control blood to mimic the remo val of the septic blood components, and (3) group C (n = 7); vehicle pretre ated lungs reperfused with normal blood as a control. Blood gas exchange, s hunt fraction (Qs/Qt), alveolar-arterial oxygen gradient (A-aDO(2),), and v ariables for lung mechanics were measured. Leukosequestration was quantifie d with a myeloperoxidase (MPO) assay. The pO(2) (mm Hg) values at 90 min af ter reperfusion in groups S, N, and C were 67.8 +/- 7.0*, 85.2 +/- 9.2, and 90.1 +/- 7.5, respectively (*p < 0.05; vs. group N and C). In addition to pO(2), A-aDO(2) and Qs/Qt significantly deteriorated in group S. MPO activi ty in the lungs after LPS infusion was significantly higher than that after vehicle infusion (1.7 +/- 0.3 vs. 0.12 +/- 0.04 units/g tissue; p < 0.001) . Subsequent reperfusion with LPS+ blood (group S) increased MPO activity t o 3.1 +/- 0.6 (p < 0.05), but reperfusion with normal blood (group N) cause d a significant decrease to 1.1 +/- 0.2 (p < 0.05). MPO activity in group C did not significantly change compared with those after vehicle infusion. R eperfusion with control blood normalized lung function compromised by pretr eatment with LPS and significantly reduced leukosequestration. These result s favor the possibility that the removal of LPS+ blood components may elimi nate septic lung injury.