Evaluation of the cardiac actin gene in Doberman Pinschers with dilated cardiomyopathy

Citation
Km. Meurs et al., Evaluation of the cardiac actin gene in Doberman Pinschers with dilated cardiomyopathy, AM J VET RE, 62(1), 2001, pp. 33-36
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
AMERICAN JOURNAL OF VETERINARY RESEARCH
ISSN journal
0002-9645 → ACNP
Volume
62
Issue
1
Year of publication
2001
Pages
33 - 36
Database
ISI
SICI code
0002-9645(200101)62:1<33:EOTCAG>2.0.ZU;2-U
Abstract
Objective-To evaluate the coding region of the cardiac actin gene in Doberm an Pinschers with dilated cardiomyopathy (DCM) for mutations that could be responsible for the development of the condition Animals-28 dogs (16 Doberman Pinschers with DCM and 12 mixed-breed control dogs). Procedure-Ten milliliters of blood was collected from each dog for DNA extr action. Polymerase chain reaction (PCR) primers were designed to amplify canine exo nic regions, using the sequences of exons 2 to 6 of the cardiac actin gene. Single-stranded conformational polymorphism analysis was performed for eac h exon with all samples. Autoradiographs were analyzed for banding patterns specific to affected dogs. The DNA sequencing was performed on a selected group of affected and control dogs. Results-Molecular analysis of exons 2 To 6 of the cardiac actin gene did no t reveal any differences in base pairs between affected dogs and control do gs selected for DNA evaluation. Conclusions-Mutations in exons 5 and 6 of the cardiac actin gene that have been reported in humans with familial DCM do not appear to be the cause of familial DCM in Doberman Pinschers. Additionally, evaluation of exons 2 to 6 for causative mutations did not reveal a cause for inherited DCM in these Doberman Pinschers. Although there is evidence that DCM in Doberman Pinsch ers is an inherited problem, a molecular basis for this condition remains u nresolved. Evaluation of other genes coding for cytoskeletal proteins is wa rranted.