Affinity labeling of methyltransferase MvaI by DNA duplexes containing oxid
ized 2'-O-beta -D-ribofuranosylcytidine or 1-(beta -D-galactopyranosyl)thym
ine residues was performed. Partial chemical hydrolysis of the covalently b
ound methylase in the conjugates with the dialdehyde-containing DNA allowed
us to determine the amino acid region in the C terminus of methylase MvaI
that interacts with DNA.