Dominant effector genetics in mammalian cells

Citation
X. Xu et al., Dominant effector genetics in mammalian cells, NAT GENET, 27(1), 2001, pp. 23-29
Citations number
47
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
NATURE GENETICS
ISSN journal
1061-4036 → ACNP
Volume
27
Issue
1
Year of publication
2001
Pages
23 - 29
Database
ISI
SICI code
1061-4036(200101)27:1<23:DEGIMC>2.0.ZU;2-C
Abstract
We have expressed libraries of peptides in mammalian cells to select for tr ans-dominant effects on intracellular signaling systems. As an example-and to reveal pharmacologically relevant points in pathways that lead to Taxol resistance-we selected for peptide motifs that confer resistance to Taxol-i nduced cell death. Of several peptides selected, one, termed RGP8.5, was li nked to upregulation of expression of the gene ABCB1 (also known as MDR1, f or multiple drug resistance) in HeLa cells. Our data indicate that trans-do minant effector peptides can point to potential mechanisms by which signali ng systems operate. Such tools may be useful in functional genomic analysis of signaling pathways in mammalian disease processes.