Viable rabbits derived from reconstructed oocytes by germinal vesicle transfer after intracytoplasmic sperm injection (ICSI)

Citation
Gp. Li et al., Viable rabbits derived from reconstructed oocytes by germinal vesicle transfer after intracytoplasmic sperm injection (ICSI), MOL REPROD, 58(2), 2001, pp. 180-185
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
MOLECULAR REPRODUCTION AND DEVELOPMENT
ISSN journal
1040-452X → ACNP
Volume
58
Issue
2
Year of publication
2001
Pages
180 - 185
Database
ISI
SICI code
1040-452X(200102)58:2<180:VRDFRO>2.0.ZU;2-7
Abstract
Abnormal oocyte spindle due to the improper function of ooplasm is associat ed with female infertility of advanced maternal age. A possible way to over come this problem is to transfer an oocyte germinal vesicle (GV) which cont ains genetic materials of a patient with a history of poor embryo developme nt to the cytoplast from a donor oocyte. Here we demonstrate that GV transf er is feasible using a rabbit model. When the GVs were transferred to auto- or heterocytoplasts of GV stage oocytes, around 80% of the reconstructed o ocytes could mature in vitro and 7.1-9.4% of the oocytes developed to blast ocyst stage after intracytoplasmic sperm injection (ICSI). Transfer of 93 f ertilized eggs reconstructed via GV transfer into six recipients resulted i n two live offspring. Results of this experiment indicate that GV transfer can potentially become a new approach in treatment of infertility because o f advanced maternal age. (C) 2001 Wiley-Liss, Inc.