Effect of endothelin-1 (1-31) on p38 mitogen-activated protein kinase in cultured human mesangial cells

Citation
D. Inui et al., Effect of endothelin-1 (1-31) on p38 mitogen-activated protein kinase in cultured human mesangial cells, LIFE SCI, 68(6), 2000, pp. 635-645
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
LIFE SCIENCES
ISSN journal
0024-3205 → ACNP
Volume
68
Issue
6
Year of publication
2000
Pages
635 - 645
Database
ISI
SICI code
0024-3205(200012)68:6<635:EOE(OP>2.0.ZU;2-0
Abstract
It was reported that human chymase cleaves big endothelins (ETs) at the Tyr (31)-Gly(32) bond and produces 31-amino acid ETs(1-31). In this study, we i nvestigated the effect of ET-1(1-31) on p38 mitogen-activated protein kinas e (p38-MAPK) activity in human mesangial cells (HMCs). By measuring the kin ase activity, we demonstrated that ET-1(1-31) activated the p38-MAPK dose-d ependently (10(-9) M to 10(-7) M), which was inhibited by SB203580. The p38 -MAPK activation induced by ET-1(1-31) peaked at 10 minutes. BQ123 almost a bolished ET-1(1-31)-induced p38-MAPK activation, whereas BQ788 failed to in hibit it. These findings suggest that the stimulatory effect of ET-1(1-31) on p38-MAPK activation is mediated through ETA or ETA-like receptor. In con clusion, ET-1(1-31) induced increase in p38-MAPK activation in cultured HMC s. (C) 2000 Elsevier Science Inc. All rights reserved.