Quantitative determination of L-775,606, a selective 5-hydroxytryptamine 1D agonist, in rat plasma using automated sample preparation and detection by liquid chromatography-tandem mass spectrometry

Citation
Kl. Locker et al., Quantitative determination of L-775,606, a selective 5-hydroxytryptamine 1D agonist, in rat plasma using automated sample preparation and detection by liquid chromatography-tandem mass spectrometry, J CHROMAT B, 750(1), 2001, pp. 13-23
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF CHROMATOGRAPHY B
ISSN journal
1387-2273 → ACNP
Volume
750
Issue
1
Year of publication
2001
Pages
13 - 23
Database
ISI
SICI code
1387-2273(20010105)750:1<13:QDOLAS>2.0.ZU;2-G
Abstract
L-775,606 is under investigation as a selective 5-hydroxytryptamine 1D agon ist for the treatment of migraine. A reliable and sensitive method for the analysis of L-775,606 in plasma was required in order to support preclinica l evaluation of this compound. A semi-automated sample preparation method u sing the Beekman Biomek 2000 workstation to perform all liquid handling tas ks has been established. The sample analysis was performed using HPLC-MS-MS with a cycle time of 3.5 min per sample. Intra- and inter-day assay accura cy and precision are excellent with a calibration range of 1-2000 ng/ml and a reproducible limit of quantification of 1 ng/ml. (C) 2001 Elsevier Scien ce B.V. All rights reserved.