Monitoring human serum transferrin by capillary zone electrophoresis with end-column amperometric detection

Citation
Q. Dong et al., Monitoring human serum transferrin by capillary zone electrophoresis with end-column amperometric detection, ELECTROPHOR, 22(1), 2001, pp. 128-133
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
ELECTROPHORESIS
ISSN journal
0173-0835 → ACNP
Volume
22
Issue
1
Year of publication
2001
Pages
128 - 133
Database
ISI
SICI code
0173-0835(200101)22:1<128:MHSTBC>2.0.ZU;2-G
Abstract
Capillary zone electrophoresis was employed for the determination of human serum transferrin using end-column amperometric detection with a carbon fib er microelectrode at a constant potential of 1.9 V vs. saturated calomel el ectrode (SCE). The optimum conditions of separation and detection are 7.5 x 10(-4) mol/L Tris-3.44 x 10(-4) mol/L HCl for the buffer solution, 20 kV f or the separation voltage, 5 kV and 10 s for the injection voltage and the injection time, respectively. The limit of detection is 6.7 x 10(-8) mol/L or 440 amol (S/N = 2). The relative standard deviations are 0.67% for the m igration time and 1.5% for the electrophoretic peak current. The method was applied to the determination of transferrin in human serum. The recovery i s between 93-104%.