Comparison of specific serological assays for diagnosing human herpesvirus6 infection after liver transplantation

Citation
T. Yoshikawa et al., Comparison of specific serological assays for diagnosing human herpesvirus6 infection after liver transplantation, CL DIAG LAB, 8(1), 2001, pp. 170-173
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
ISSN journal
1071-412X → ACNP
Volume
8
Issue
1
Year of publication
2001
Pages
170 - 173
Database
ISI
SICI code
1071-412X(200101)8:1<170:COSSAF>2.0.ZU;2-E
Abstract
Cross-reactivity between human herpesvirus 6 (HHV-6) and human herpesvirus 7 (HHV-7) antibodies and the reliability of specific serological assays wer e analyzed for 12 patients with concurrent HHV-6 and HHV-7 antibody respons es after transplantation with a liver from a living relative by using an im munofluorescence assay (IFA). A neutralizing antibody titer assay (NT) and an immunoblot assay (IB) designed to detect immunoglobulin M (IgM) antibody to the HHV-6 immunodominant 101-kDa protein were compared in the diagnosis of an active HHV-6 infection. A total of 9 of 12 patients demonstrated con current HHV-6 and HHV-7 antibody responses, including increased Ige titers and/or the presence of IgM by IFA, and were thus analyzed for cross-reactiv e antibody to heterologous virus. The average percentages of residual antib ody to HHV-6 and HHV-7 after absorption with HHV-6 antigen were 32.6% (rang e, 6 to 50%) and 55.6% (range, 35 to 100%), respectively. All 12 patients w ere subsequently analyzed for HHV-6 antibody by using IB and NT, IB detecte d IgM antibody to the 101-kDa protein in 75% (9 of 12) of the recipients. A significant rise in the NT antibody titer was detected in the same nine sa mples. However, HHV-6 DNA was detected by PCR in only five of nine plasma s amples collected from recipients with a specific serologic response against HHV-6.