H. Kuwahara et al., Transfer of platelet-derived growth factor-be gene by gene gun increases contraction of collagen lattice by fibroblasts in diabetic and non-diabetic human skin, SC J PLAST, 34(4), 2000, pp. 301-307
SCANDINAVIAN JOURNAL OF PLASTIC AND RECONSTRUCTIVE SURGERY AND HAND SURGERY
We have used an in vitro model of wound contraction, the fibroblast-populat
ed collagen lattice, to examine the effect of platelet-derived growth facto
r BE (PDGF-BB) and PDGF-BB gene transfer by gene gun on the contraction of
lattices composed of either diabetic or non-diabetic human fibroblasts. The
area of collagen lattice and DNA synthesis were measured in 12 specimens.
There were significant increases in lattice contraction with increasing dos
es of PDGF-BB and fibroblasts transfected with the PDGF-BB gene compared wi
th control (p < 0.01). DNA synthesis of the non-diabetic and diabetic fibro
blast lattices showed significantly increased incorporation of tritiated th
ymidine with increasing doses of PDGF-BB and fibroblasts transfected with t
he PDGF-BB compared with controls (p < 0.05) The effect of PDGF-BB gene tra
nsfer on diabetic and non-diabetic fibroblasts was similar to that of 20 ng
/ml or less of PDGF-BB.