Transfer of platelet-derived growth factor-be gene by gene gun increases contraction of collagen lattice by fibroblasts in diabetic and non-diabetic human skin

Citation
H. Kuwahara et al., Transfer of platelet-derived growth factor-be gene by gene gun increases contraction of collagen lattice by fibroblasts in diabetic and non-diabetic human skin, SC J PLAST, 34(4), 2000, pp. 301-307
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Surgery
Journal title
SCANDINAVIAN JOURNAL OF PLASTIC AND RECONSTRUCTIVE SURGERY AND HAND SURGERY
ISSN journal
0284-4311 → ACNP
Volume
34
Issue
4
Year of publication
2000
Pages
301 - 307
Database
ISI
SICI code
0284-4311(200012)34:4<301:TOPGFG>2.0.ZU;2-C
Abstract
We have used an in vitro model of wound contraction, the fibroblast-populat ed collagen lattice, to examine the effect of platelet-derived growth facto r BE (PDGF-BB) and PDGF-BB gene transfer by gene gun on the contraction of lattices composed of either diabetic or non-diabetic human fibroblasts. The area of collagen lattice and DNA synthesis were measured in 12 specimens. There were significant increases in lattice contraction with increasing dos es of PDGF-BB and fibroblasts transfected with the PDGF-BB gene compared wi th control (p < 0.01). DNA synthesis of the non-diabetic and diabetic fibro blast lattices showed significantly increased incorporation of tritiated th ymidine with increasing doses of PDGF-BB and fibroblasts transfected with t he PDGF-BB compared with controls (p < 0.05) The effect of PDGF-BB gene tra nsfer on diabetic and non-diabetic fibroblasts was similar to that of 20 ng /ml or less of PDGF-BB.