Relationship of cytokeratin 20 and CD44 protein expression with WHO/ISUP grade in pTa and pT1 papillary urothelial neoplasia

Citation
S. Desai et al., Relationship of cytokeratin 20 and CD44 protein expression with WHO/ISUP grade in pTa and pT1 papillary urothelial neoplasia, MOD PATHOL, 13(12), 2000, pp. 1315-1323
Citations number
49
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Journal title
MODERN PATHOLOGY
ISSN journal
0893-3952 → ACNP
Volume
13
Issue
12
Year of publication
2000
Pages
1315 - 1323
Database
ISI
SICI code
0893-3952(200012)13:12<1315:ROC2AC>2.0.ZU;2-9
Abstract
The aim of this study was to assess the relationship of immunoreactivity of cytokeratin 20 (CK20) and CD44 across the spectrum of urothelial neoplasia using the WHO/ISUP consensus classification A total of 120 papillary uroth elial pTa and pTl tumors (8 papillomas, 8 neoplasms of low malignant potent ial, and 42 low-grade and 62 high-grade carcinomas) were immunostained by u sing CK20 and CD44 antibodies. The relationships of tumor grade, pathologic stage, recurrences, and progression In stage with CK20 and CD44 immunoreac tivity were assessed, WHO/ISUP grade correlated with tumor stage (P < 0.005 ), recurrence (P = 0.02), and progression in stage (P = 0.031). Normal urot helium showed CK20 immunoreactivity restricted to a few umbrella cells. Exp ression of CD44 in normal urothelium was restricted to the basal cell layer . Loss of CD44 immunoreactivity and increasing CK20 positivity were signifi cantly associated with increasing tumor grade and stage (P < 0.005). An inv erse relationship was observed in the staining patterns of CK20 and CD44 wi thin individual cases, as wed as In the aggregate data, with 79.2% of tumor s with CD44 loss showing CK20 positivity (P < 0.001). In conclusion, CK20 a nd CD44 immunoreactivity are significantly related to the WHO/ISUP grade an d to each other, and our data suggest their potential combined utility in p redicting biologic behavior In patients with papillary urothelial pTa and p Tl neoplasms.