In situ CD14 expression in biliary atresia: Comparison between early and late stages

Citation
Afku. Ahmed et al., In situ CD14 expression in biliary atresia: Comparison between early and late stages, J PED SURG, 36(1), 2001, pp. 240-243
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pediatrics
Journal title
JOURNAL OF PEDIATRIC SURGERY
ISSN journal
0022-3468 → ACNP
Volume
36
Issue
1
Year of publication
2001
Pages
240 - 243
Database
ISI
SICI code
0022-3468(200101)36:1<240:ISCEIB>2.0.ZU;2-W
Abstract
Purpose: The aim of this study was to compare the in situ expression of CD1 4 between early and late stage of biliary atresia (BA) to determine if a ti me course of CD14 expression exists in BA. Methods: Immunohistochemical analysis of membrane-bound CD14 expression was carried out in periodate-lysine-paraformaldehyde (PLP)-fixed frozen sectio ns from 9 early- (obtained during Kasai procedure) and 6 late- (obtained du ring liver transplantation) stage cases of BA, Normal liver (n = 3) and cho ledochal cysts (n = 5) served as normal controls and disease controls respe ctively. Results: In the early stage, 6 patients (66.66%) showed extensive CD14 expr ession (grade 3 [G(3)], more than 50% positive cells), whereas no CD14-posi tive cells could be detected in 4 patients (66.66%) in the late stage. In b oth stages, most of the positive cells were observed in the parenchyma of t he hepatic lobules where Kupffer cells and sinusoidal endothelial cells sta ined positive. Arterial and venous endothelium, bile duct cells, and hepato cytes were negative for CD14, Double immunohistochemistry in the early stag e showed a higher colocalization rate of CD14 and CD68 in the sinusoidal lo cations (33.69 +/- 9.270% [mean +/- SEM]) than in the portal tract (7.6 +/- 4.64% [mean +/- SEM]; P < .05). Similar pattern of colocalization also was observed in the late stage. In the normal controls no expression of CD14 c ould be detected, whereas in the disease controls only 1 case showed mild e xpression (grade 1 [G(1)], 1% to 10% positive cells) and the rest showed no expression of CD14. Conclusion: These results suggest that CD14 expression in BA is a dynamic p henomenon having time-related change with overexpression in the early stage and reduced expression in the late stage. J Pediatr Surg 36:240-243, Copyr ight (C) 2001 by W.B. Saunders Company.