Resveratrol inhibits interleukin-6 production in cortical mixed glial cells under hypoxia/hypoglycemia followed by reoxygenation

Citation
Mj. Wang et al., Resveratrol inhibits interleukin-6 production in cortical mixed glial cells under hypoxia/hypoglycemia followed by reoxygenation, J NEUROIMM, 112(1-2), 2001, pp. 28-34
Citations number
46
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
JOURNAL OF NEUROIMMUNOLOGY
ISSN journal
0165-5728 → ACNP
Volume
112
Issue
1-2
Year of publication
2001
Pages
28 - 34
Database
ISI
SICI code
0165-5728(20010101)112:1-2<28:RIIPIC>2.0.ZU;2-Q
Abstract
Reactive oxygen intermediates (ROIs) are important mediators of a variety o f pathological processes, including inflammation and ischemia/reperfusion i njury. Cytokines and chemokines are detected at mRNA level in human and ani mal ischemic brains. This suggests that hypoxia/reoxygenation may induce cy tokine production through generation of ROIs. In this study, we investigate d the cytokine induction and inhibition by antioxidants in rat cortical mix ed glial cells exposed to in vitro ischemia-like insults (hypoxia plus gluc ose deprivation). The results showed that interleukin-6 (IL-6) mRNA and pro tein, but not tumor necrosis factor-alpha (TNF-alpha) or interleukin-1 beta (IL-1 beta), were induced during hypoxia/hypoglycemia followed by reoxygen ation in the mixed glial cells. The accumulation of IL-6 mRNA was induced a s early as 15 min after hypoxia/hypoglycemia and its level was further incr eased after subsequent reoxygenation. Among the antioxidants studied, only resveratrol suppressed IL-6 gene expression and protein secretion in mixed glial cultures under hypoxia/hypoglycemia followed by reoxygenation. These findings suggest that resveratrol might be useful in treating ischemic-indu ced inflammatory processes in stroke. (C) 2001 Elsevier Science B.V. All ri ghts reserved.