Effect of methylprednisolone on the kinetics of cytokines and liver function of regenerating liver in rats

Citation
T. Fujioka et al., Effect of methylprednisolone on the kinetics of cytokines and liver function of regenerating liver in rats, HEPATOL RES, 19(1), 2001, pp. 60-73
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Gastroenerology and Hepatology
Journal title
HEPATOLOGY RESEARCH
ISSN journal
1386-6346 → ACNP
Volume
19
Issue
1
Year of publication
2001
Pages
60 - 73
Database
ISI
SICI code
1386-6346(200101)19:1<60:EOMOTK>2.0.ZU;2-Y
Abstract
The participation of cytokines in the early stage mechanism of hepatocyte p roliferation has already attracted attention. We investigated the effect of methylprednisolone (MDS). which inhibits the inflammatory response, given before and after a 70% partial hepatectomy in rats on the kinetics of tumor necrosis factor-alpha and Interleukin-6, liver cell function and the rate of liver regeneration. Serum Interleukin-6 levels of the MDS groups were si gnificantly lower than those of the control group. Serum alanine aminotrans ferase, aspartate aminotransferase and hyaluronic acid levels were also sig nificantly decreased, however, the serum albumin level showed high values i n the MDS groups. In the MDS groups, MIB-5 labeling indices, a novel antibo dy reactive with the equivalent Ki-67 protein, which detects immunohistoche mically all active parts of the cell cycle in the rat liver, were more pron ounced than in the control group at an earlier time. However, in regard to 5-bromo-2-deoxyuridine (BrdU), there were no significant differences among the three groups. There were no differences in residual liver weight/body w eight between the three groups after 336 h. In our study, MDS administratio n before or after a 70% partial hepatectomy decreased serum Interleukin-6 l evels, and inhibited hepatic dysfunction. Therefore, we considered that ben eficial effects of physiological doses of MDS in the peri-operative period should be confirmed in humans. (C) 2001 Elsevier Science Ireland Ltd. All r ights reserved.