Aberrant expression of beta-catenin and mutation of exon 3 of the beta-catenin gene in renal and urothelial carcinomas

Citation
Xd. Zhu et al., Aberrant expression of beta-catenin and mutation of exon 3 of the beta-catenin gene in renal and urothelial carcinomas, PATHOL INT, 50(12), 2000, pp. 945-952
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
PATHOLOGY INTERNATIONAL
ISSN journal
1320-5463 → ACNP
Volume
50
Issue
12
Year of publication
2000
Pages
945 - 952
Database
ISI
SICI code
1320-5463(200012)50:12<945:AEOBAM>2.0.ZU;2-B
Abstract
The present study attempted to clarify the significance of aberrant express ion of beta -catenin protein and mutation of exon 3 of the beta -catenin ge ne in renal and urothelial carcinogenesis. beta -Catenin expression was exa mined immunohistochemically and mutation of the beta -catenin gene was anal yzed by polymerase chain reaction-single strand conformation polymorphism ( SSCP) and direct sequencing. beta -Catenin immunoreactivity was observed at the cell membrane in all 30 renal cell carcinomas (RCC) examined, and no R CC showed a mobility-shifted SSCP band. Of 46 transitional cell carcinomas (TCC) examined, there was reduced expression of beta -catenin, as compared with its expression in non-cancerous transitional epithelium, in 22 cases ( 48%) and beta -catenin accumulation in the nucleus in five cases (11%). Of four renal pelvis TCC examined, point mutation of exon 3 of the beta -caten in gene at codon 45 resulting in amino acid substitution (Ser to Phe) was d etected in one (25%). The incidence of reduced expression of beta -catenin correlated significantly with the growth pattern (superficial type vs invas ive type) of TCC (P < 0.05). These data indicate that: (1) aberrant beta -c atenin expression may be at least partly involved in urothelial carcinogene sis, but less significantly so in renal carcinogenesis, and (2) it may be a ssociated with the progression of TCC showing invasive growth.