Agrin controls synaptic differentiation in hippocampal neurons

Citation
Cm. Bose et al., Agrin controls synaptic differentiation in hippocampal neurons, J NEUROSC, 20(24), 2000, pp. 9086-9095
Citations number
71
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
JOURNAL OF NEUROSCIENCE
ISSN journal
0270-6474 → ACNP
Volume
20
Issue
24
Year of publication
2000
Pages
9086 - 9095
Database
ISI
SICI code
0270-6474(200012)20:24<9086:ACSDIH>2.0.ZU;2-V
Abstract
Agrin controls the formation of the neuromuscular junction. Whether it regu lates the differentiation of other types of synapses remains unclear. There fore, we have studied the role of agrin in cultured hippocampal neurons. Sy naptogenesis was severely compromised when agrin expression or function was suppressed by antisense oligonucleotides and specific antibodies. The effe cts of antisense oligonucleotides were found to be highly specific because they were reversed by adding recombinant agrin and could not be detected in cultures from agrin-deficient animals. Interestingly, the few synapses for med in reduced agrin conditions displayed diminished vesicular turnover, de spite a normal appearance at the EM level. Thus, our results demonstrate th e necessity of agrin for synaptogenesis in hippocampal neurons.