Stimulatory effect of zinc and growth factor on bone protein component in newborn rats: Enhancement with zinc and insulin-like growth factor-I

Citation
Zj. Ma et M. Yamaguchi, Stimulatory effect of zinc and growth factor on bone protein component in newborn rats: Enhancement with zinc and insulin-like growth factor-I, INT J MOL M, 7(1), 2001, pp. 73-78
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research General Topics
Journal title
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
ISSN journal
1107-3756 → ACNP
Volume
7
Issue
1
Year of publication
2001
Pages
73 - 78
Database
ISI
SICI code
1107-3756(200101)7:1<73:SEOZAG>2.0.ZU;2-J
Abstract
effect of zinc and growth factor on bone protein component in newborn rats was investigated. The characterization of protein component in the femoral- diaphyseal and metaphyseal tissue of newborn rats (3-35 days old) was exami ned using sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysi s. The diaphyseal and metaphyseal tissues of 7 days-old rats were cultured for 24 or 48 h in a medium containing either vehicle, zinc sulfate (10(-4) M) or dipicolinate (10(-3) M), a chelator of zinc ion, in the presence or a bsence of insulin-like growth factor-I (IGF-I; 10(-8) M) or transforming gr owth factor-beta (TFG-beta; 10(-10) M) with an effective concentration. Man y cellular protein molecules were present in the diaphyseal and metaphyseal tissues; potent bands were seen in protein molecules with about 66 and 46 kDa. Protein molecule of about 66 kDa was greatly released in the medium cu ltured with the diaphyseal and metaphyseal tissues. This protein in the med ium was increased by culture with zinc, IGF-I or TGF-beta. Total protein co ntent in the medium cultured with the diaphyseal and metaphyseal tissues wa s significantly increased in the presence of zinc, IGF-I or TGF-beta. The I GF-I-increased medium protein content was significantly enhanced by zinc. T his enhancing effect was not seen in TGF-beta. Alkaline phosphatase activit y and deoxyribonucleic acid (DNA) content in the diaphyseal and metaphyseal tissues was significantly increased by culture with zinc, IGF-I or TGF-bet a. The effect of IGF-I was significantly enhanced by zinc, while it was not found in TGF-beta. The effect of IGF-I or TGF-beta in increasing the bone components was seen in the presence of dipicolinate. This study demonstrate s that zinc, like IGF-I and TGF-beta, can increase protein components in th e femoral-diaphyseal and metaphyseal tissues of new- born rats. Zinc may es pecially play a role in bone growth in collaboration with TGF-I.