In vivo gene electroporation confers nutritionally-regulated foreign gene expression in the liver

Muramatsu, T Ito, N Tamaoki, N Oda, H Park, HM

T. Muramatsu et al., In vivo gene electroporation confers nutritionally-regulated foreign gene expression in the liver, INT J MOL M, 7(1), 2001, pp. 61-66

34

INGLESE

Article

Medical Research General Topics

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE

1107-3756 → ACNP

7

1

2001

61 - 66

ISI

1107-3756(200101)7:1<61:IVGECN>2.0.ZU;2-V

Whether or not nutritionally-regulated foreign gene expression in vivo is a chievable was examined in mouse liver after in vivo gene transfer by electr oporation (EP). Electric pulses were applied to a left liver lobe immediate ly after injection of a luciferase reporter gene driven by the liver-type p hosphoenolpyruvate carboxykinase (PFPCK) gene promoter. Cooling treatments especially with solid carbon dioxide in the transfection site prior to the in vivo gene EP increased reporter gene expression by a factor of 100. Body bioluminescence imaging also confirmed strong expression of the in vivo tr ansferred reporter gene in a transfected area of the liver. Fasting conferr ed a 13-fold increase in the reporter gene expression in vivo in the liver when driven by the liver-type PEPCK promoter, whereas virtually no inductio n was found either by the SV40 promoter or by the same PEPCK promoter in th e muscle when the mice were fasted. The administration of cAMP mimicked the fasting-induced reporter gene expression by the PEPCK promoter in the live r of fed mice. These results implicate that nutritionally-regulated foreign gene expression in vivo is attainable at least locally in the liver by a s imple and convenient non-viral gene EP method.