Stage-associated expression of ceramide structures in glycosphingolipids from the human trematode parasite Schistosoma mansoni

Citation
M. Wuhrer et al., Stage-associated expression of ceramide structures in glycosphingolipids from the human trematode parasite Schistosoma mansoni, BBA-GEN SUB, 1524(2-3), 2000, pp. 155-161
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
ISSN journal
0304-4165 → ACNP
Volume
1524
Issue
2-3
Year of publication
2000
Pages
155 - 161
Database
ISI
SICI code
0304-4165(200012)1524:2-3<155:SEOCSI>2.0.ZU;2-5
Abstract
Glycosphingolipids of Schistosoma mansoni adults, cercariae and eggs compri se ceramide monohexosides (CMH) with glucose or galactose and ceramide dihe xosides (CDH) with the schistosome-specific structure GalNAc(beta1-4)Glc(l- l)ceramide. Ceramide analysis revealed C18- and C20-phytosphingosines in eg g CMH, C18-sphinganine as well as C18-, C19- and C20-phytosphingosines in c ercarial CMH, and C18- and C20-phytosphingosines as well as C18-sphingosine and C18-sphinganine in adult CMH. For all three life cycle stages, the pre dominant fatty acid was C16h:0. As a characteristic feature, a range of sat urated, unsaturated and hydroxylated long-chain fatty acids with 24-28 carb on atoms were additionally found in minor cercarial CMH species. The corres ponding ceramides represented major constituents in cercarial CDH, while ad ult and egg CDH were dominated by ceramides with short fatty acid chains. T he resultant ceramide patterns could be correlated with the differential ex pression of carbohydrate antigens on schistosomal glycolipids at various st ages. A possible impact of ceramide structure on the biosynthesis of the ca rbohydrate moieties is discussed. (C) 2000 Elsevier Science B.V. All rights reserved.