Z. Kaleem et al., Critical analysis and diagnostic usefulness of limited immunophenotyping of B-cell non-Hodgkin lymphomas by flow cytometry, AM J CLIN P, 115(1), 2001, pp. 136-142
Citations number
12
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
We report the sensitivity, specificity positive predictive value (PPV), and
negative predictive value (NPV) of various immunophenotypes characteristic
of each class of B-cell non-Hodgkin lymphoma (NHL) based on analysis of 35
2 morphologically well-characterized B-cell NHLs and 175 benign lymph nodes
(LNs) using 2-color flow cytometry. All B-cell NHLs that exhibited a chara
cteristic immunophenotype (except diffuse large B-cell lymphoma) had a high
NPK The immunophenotypes of small lymphocytic lymphoma and mantle cell lym
phoma showed high specificity but only small lymphocytic lymphoma also show
ed a high PPV. One third of follicular lymphomas coexpressed CD23 and CD10.
Diffuse large B-cell NHL showed no consistent immunophenotype. About 90% o
f all benign LNs expressed no substantial amounts of CD5, CD10, or CD23. Mo
st benign LNs also failed to express substantial amounts of immunoglobulin
heavy chains. In contrast, about 90% of NHLs showed expression of 1 or 2 he
avy chains. The expression pattern of immunoglobulin light chains was not f
ound helpful in favoring one lymphoma type over another The usefulness of e
ach immunophenotype for each lymphoma group is of particular diagnostic imp
ortance in limited specimens, such as fine-needle aspiration biopsies, smal
l core biopsies, body effusions, extranodal sites, and nodal tissues with v
arious artifacts.