Critical analysis and diagnostic usefulness of limited immunophenotyping of B-cell non-Hodgkin lymphomas by flow cytometry

Citation
Z. Kaleem et al., Critical analysis and diagnostic usefulness of limited immunophenotyping of B-cell non-Hodgkin lymphomas by flow cytometry, AM J CLIN P, 115(1), 2001, pp. 136-142
Citations number
12
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Volume
115
Issue
1
Year of publication
2001
Pages
136 - 142
Database
ISI
SICI code
Abstract
We report the sensitivity, specificity positive predictive value (PPV), and negative predictive value (NPV) of various immunophenotypes characteristic of each class of B-cell non-Hodgkin lymphoma (NHL) based on analysis of 35 2 morphologically well-characterized B-cell NHLs and 175 benign lymph nodes (LNs) using 2-color flow cytometry. All B-cell NHLs that exhibited a chara cteristic immunophenotype (except diffuse large B-cell lymphoma) had a high NPK The immunophenotypes of small lymphocytic lymphoma and mantle cell lym phoma showed high specificity but only small lymphocytic lymphoma also show ed a high PPV. One third of follicular lymphomas coexpressed CD23 and CD10. Diffuse large B-cell NHL showed no consistent immunophenotype. About 90% o f all benign LNs expressed no substantial amounts of CD5, CD10, or CD23. Mo st benign LNs also failed to express substantial amounts of immunoglobulin heavy chains. In contrast, about 90% of NHLs showed expression of 1 or 2 he avy chains. The expression pattern of immunoglobulin light chains was not f ound helpful in favoring one lymphoma type over another The usefulness of e ach immunophenotype for each lymphoma group is of particular diagnostic imp ortance in limited specimens, such as fine-needle aspiration biopsies, smal l core biopsies, body effusions, extranodal sites, and nodal tissues with v arious artifacts.