Cooperative transformation and coexpression of bovine papillomavirus type 1 E5 and E7 proteins

Citation
J. Bohl et al., Cooperative transformation and coexpression of bovine papillomavirus type 1 E5 and E7 proteins, J VIROLOGY, 75(1), 2001, pp. 513-521
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGY
ISSN journal
0022-538X → ACNP
Volume
75
Issue
1
Year of publication
2001
Pages
513 - 521
Database
ISI
SICI code
0022-538X(200101)75:1<513:CTACOB>2.0.ZU;2-U
Abstract
Productively infected bovine fibropapillomas were examined for bovine papil lomavirus type 1 (BPV-1) E7 localization. BPV-1 E7 was observed in the cyto plasm of basal and lower spinous epithelial cells, coexpressed in the cytop lasm of basal cells with the E5 oncoprotein. E7 aas also observed in nucleo li throughout the basal and spinous layers but not in the granular cell lay er. Ectopic expression of E7 in cultured epithelial cells gave rise to loca lization similar to that seen in productive fibropapillomas, with cytoplasm ic and nucleolar expression observed. Consistent with the coexpression of E 7 and E5 in basal keratinocytes, BPV-1 E7 cooperated with E5 as well as E6 in an anchorage independence transformation assay. While E5 is expressed in both basal and superficial differentiating keratinocytes, BPV-1 E7 is only observed in basal and lower spinous epithelial cells. Therefore, BPV-1 E7 may serve to modulate the cellular response of basal epithelial cells to E5 expression.