Finding nuclear localization signals

Citation
M. Cokol et al., Finding nuclear localization signals, EMBO REP, 1(5), 2000, pp. 411-415
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
EMBO REPORTS
ISSN journal
1469-221X → ACNP
Volume
1
Issue
5
Year of publication
2000
Pages
411 - 415
Database
ISI
SICI code
1469-221X(200011)1:5<411:FNLS>2.0.ZU;2-8
Abstract
A variety of nuclear localization signals (NLSs) are experimentally known a lthough only one motif was available for database searches through PROSITE. We initially collected a set of 91 experimentally verified NLSs from the l iterature. Through iterated 'in silico mutagenesis' we then extended the se t to 214 potential NLSs. This final set matched in 43% of all known nuclear proteins and in no known non-nuclear protein. We estimated that >17% of al l eukaryotic proteins may be imported into the nucleus. Finally, we found a n overlap between the NLS and DNA-binding region for 90% of the proteins fo r which both the NLS and DNA-binding regions were known. Thus, evolution se emed to have used part of the existing DNA-binding mechanism when compartme ntalizing DNA-binding proteins into the nucleus. However, only 56 of our 21 4 NLS motifs overlapped with DNA-binding regions. These 56 NLSs enabled a d e novo prediction of partial DNA-binding regions for similar to 800 protein s in human, fly, worm and yeast.