Nitric oxide (NO) and interleukin-1 beta (IL-1 beta) in follicular fluid and their correlation with fertilization and embryo cleavage

Citation
Mj. Barrionuevo et al., Nitric oxide (NO) and interleukin-1 beta (IL-1 beta) in follicular fluid and their correlation with fertilization and embryo cleavage, AM J REPROD, 44(6), 2000, pp. 359-364
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
ISSN journal
1046-7408 → ACNP
Volume
44
Issue
6
Year of publication
2000
Pages
359 - 364
Database
ISI
SICI code
1046-7408(200012)44:6<359:NO
Abstract
PROBLEM: Using an IVF model, the goal of the study was to investigate the r elationship between follicular fluid (ff) NO and IL-1 beta levels, as well as their correlation with fertilization of mature oocytes and embryo cleava ge rates. METHOD OF STUDY: Follicular fluid was collected from 17 patients at the tim e of transvaginal oocyte retrieval following controlled ovarian stimulation . Oocytes harvested from these follicles were followed through fertilizatio n and embryo cleavage. The NO metabolites nitrate/nitrite (NO3/NO2) were me asured using the Griess reaction as an indirect assessment of NO activity. IL-1 beta was measured using a high sensitivity ELISA system (Amersham, UK) . The Student's t-test was utilized for unpaired data with the means consid ered significantly different when P less than or equal to 0.05. RESULTS: Follicular fluid NO3/NO2 levels were significantly lower in follic les containing mature oocytes that fertilized (n = 30; 9.7 +/- 1.0 muM), ve rsus those that did not fertilize (n = 23; 15.4 +/- 2.4 muM; P < 0.05). Fol licles that contained oocytes that fertilized and went on to divide beyond the 6 cell stage had significantly lower ff levels of NO3/NO2 (n = 18; 7.5 +/- 0.9 <mu>M), as compared to ff that contained oocytes that did not ferti lize or failed to develop beyond the 5 cell stage (n = 35; 14.6 +/- 1.7 muM ; P < 0.01). No correlation was found between ff NO3/NO2 levels (n = 28; 13 .8 +/- 2.0 <mu>M) and ff IL-1 beta levels (n = 28; 0.5 +/- 0.08 pg/mL). An analysis of ff IL-IP levels in relation to fertilization and embryo cleavag e rates revealed no correlation. CONCLUSIONS: Lower ff NO3/NO2 levels at the time of oocyte retrieval are as sociated with adequate fertilization and embryo cleavage rates. In our IVF model, no correlation was found between ff IL-1 beta levels and ff NO3/NO2, fertilization, or embryo cleavage rates.