Identification of epididymis-specific antigens using neonatal tolerization

Citation
V. Khole et al., Identification of epididymis-specific antigens using neonatal tolerization, AM J REPROD, 44(6), 2000, pp. 350-358
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
ISSN journal
1046-7408 → ACNP
Volume
44
Issue
6
Year of publication
2000
Pages
350 - 358
Database
ISI
SICI code
1046-7408(200012)44:6<350:IOEAUN>2.0.ZU;2-S
Abstract
PROBLEM: Conventional immunization using whole sperm containing multiple an tigens as the immunogen followed by hybridoma technology usually gives anti bodies to antigens invariably of testicular origin, probably because of the strong immunogenic nature of these antigens. Therefore, an alternate appro ach of neonatal tolerization or subtractive immunization has been utilized to raise antibodies specific to epididymis by suppressing immune response t o testicular antigens. METHOD OF STUDY: Neonatal mice were tolerized with testicular sperm protein s on days 0 and 5. These animals were then immunized with epididymal sperm proteins on day 21, followed by two boosters at biweekly intervals. Sera fr om these mice were used to localize epididymis-specific antigens. RESULTS: Sera from mice that were tolerized to testicular sperm proteins an d later immunized with epididymal sperm proteins reacted only with epididym al proteins. CONCLUSION: The results of this study demonstrate that neonatal tolerizatio n with testicular sperm proteins, followed by immunization with epididymal sperm proteins, enhances the production of antibodies to proteins exclusive ly of epididymal origin.