PROBLEM: Conventional immunization using whole sperm containing multiple an
tigens as the immunogen followed by hybridoma technology usually gives anti
bodies to antigens invariably of testicular origin, probably because of the
strong immunogenic nature of these antigens. Therefore, an alternate appro
ach of neonatal tolerization or subtractive immunization has been utilized
to raise antibodies specific to epididymis by suppressing immune response t
o testicular antigens.
METHOD OF STUDY: Neonatal mice were tolerized with testicular sperm protein
s on days 0 and 5. These animals were then immunized with epididymal sperm
proteins on day 21, followed by two boosters at biweekly intervals. Sera fr
om these mice were used to localize epididymis-specific antigens.
RESULTS: Sera from mice that were tolerized to testicular sperm proteins an
d later immunized with epididymal sperm proteins reacted only with epididym
CONCLUSION: The results of this study demonstrate that neonatal tolerizatio
n with testicular sperm proteins, followed by immunization with epididymal
sperm proteins, enhances the production of antibodies to proteins exclusive
ly of epididymal origin.