PROBLEM: To determine if fete-placental tissues from gestations complicated
by pregnancy-induced hypertension (PIH) have altered expression of Fas-ass
METHOD OF STUDY: The expression of several Fas-related proteins was determi
ned in fetal membranes, decidua, and placentas obtained from PIH-affected (
n = 12, age range 32-36 weeks) and normal (n=6, age range 37-41 weeks) gest
ations. Paraffin-embedded tissue sections were stained with specific monocl
onal antibodies to Fas, Fas ligand (FasL), caspase-3, and bax.
RESULTS: We observed greater expression of Fas and FasL in amnion and decid
ua from PIH-affected gestations than in normal controls. Intense staining w
as observed only in the perivascular endothelium (caspase-3) and in decidua
l cells (bax) from PIH gestations.
CONCLUSION: Differential expression of Fas-related proteins in fetal membra
nes, decidua, and placentas from PIH-affected gestations is consistent with
increased apoptosis, and suggests activation of the Fas/FasL pathway in a