Inhibition of the antibody responses to rat blood transfusion antigens in mice by boar seminal immunosuppressive fraction

Citation
L. Veselsky et al., Inhibition of the antibody responses to rat blood transfusion antigens in mice by boar seminal immunosuppressive fraction, AM J REPROD, 44(6), 2000, pp. 325-335
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
ISSN journal
1046-7408 → ACNP
Volume
44
Issue
6
Year of publication
2000
Pages
325 - 335
Database
ISI
SICI code
1046-7408(200012)44:6<325:IOTART>2.0.ZU;2-2
Abstract
PROBLEM: Immunosuppressive fraction of boar seminal vesicle fluid (ISF) was tested to muffle primary and secondary antibody responses to xenotranfusio ns. Contemporaneously, heparin non-binding fraction of seminal plasma (H- f raction), presumed to be identical to ISF, was used to support the results. METHOD: To study their similarity, ISF and H- fraction were analyzed by hig h-performance liquid chromatography and the separated proteins by N-termina l sequencing. In sera of mice treated with ISF or H- fraction, the producti ons of antibodies against rat erythrocytes and blood serum were evaluated b y enzyme-linked immunosorbent assay (ELISA). The productions of IgM, IgA, a nd IgG subclasses were followed by sandwich ELISA. RESULTS: ISF and H- fraction were proved to be equal complexes of porcine s eminal plasma (PSP) proteins PSP I and PSP II. Both inhibited antibody resp onses to rat erythrocytes and serum and the concentrations of IgM, IgG, IgG , and IgG, after the first transfusion with a long-lasting effect. Both sup pressed the secondary antibody production if applied before the second tran sfusion. IgA and IgG, stayed uninfluenced. ISF and H- fraction had an equal immunosuppressive effect. CONCLUSIONS: ISF was characterized biochemically, found to be identical to H- fraction, and determined to be powerful in overcoming unwanted exaggerat ed antibody responses to xenotransfusion.