Localization of the pituitary adenylate cyclase-activating polypeptide receptor and its mRNA in the rat adrenal medulla

Shioda, S Shimoda, Y Hori, T Mizushima, H Ajiri, T Funahashi, H Ohtaki, K Ryushi, T

S. Shioda et al., Localization of the pituitary adenylate cyclase-activating polypeptide receptor and its mRNA in the rat adrenal medulla, NEUROSCI L, 295(3), 2000, pp. 81-84

18

INGLESE

Article

Neurosciences & Behavoir

NEUROSCIENCE LETTERS

0304-3940 → ACNP

295

3

2000

81 - 84

ISI

0304-3940(200012)295:3<81:LOTPAC>2.0.ZU;2-R

We examined the localization of the pituitary adenylate cyclase-activating peptide (PACAP) receptor (PAC1-R) and its mRNA with immunocytochemistry and in situ hybridization, respectively. PAC1-R immunoreactivity and its trans cript were detected in both chromaffin cells and ganglion cells but not det ected in the adrenal cortex. In addition, strong PAC1-R immunoreactivity wa s found beneath the plasma membrane of the immunoreactive medullary cells. Electron microscopic immunocytochemistry revealed that PAC1-R was predomina ntly expressed in adrenaline-containing cells. This report supports the not ion that PACAP is an activator and modulator of catecholamine secretion as well as synthesis in the adrenal medulla. (C) 2000 Published by Elsevier Sc ience Ireland Ltd.