Rapid staining of proteins on polyacrylamide gels and nitrocellulose membranes using a mixture of fluorescent dyes

Ganesh, G Kumar, TKS Pandian, STK Yu, C

G. Ganesh et al., Rapid staining of proteins on polyacrylamide gels and nitrocellulose membranes using a mixture of fluorescent dyes, J BIOCH BIO, 46(1-2), 2000, pp. 31-38

16

INGLESE

Article

Biochemistry & Biophysics

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS

0165-022X → ACNP

46

1-2

2000

31 - 38

ISI

0165-022X(20001120)46:1-2<31:RSOPOP>2.0.ZU;2-B

The present work describes a novel, fluorescence-based method for staining proteins on SDS-PAGE and membrane(s). In this method, proteins are stained using a mixed-dye (sulforhodamine B and 1-anilino-8-naphthalene sulfonic ac id (NH4+)) solution. The mixed-dye staining protocol can detect proteins up to a concentration of 15 ng. This method is generally applicable to all pr oteins and is more sensitive than the conventional Coomassie blue method. T he staining method is rapid and efficient. Staining-destaining of proteins using the mixed-dye protocol takes less than half an hour. Another interest ing feature of the staining protocol described here is the applicability to the staining of proteins on nitrocellulose membranes. (C) 2000 Elsevier Sc ience B.V. All rights reserved.