Extragenic suppressors of the nimX2(cdc2) mutation of Aspergillus nidulansaffect nuclear division, septation and conidiation

Citation
Sl. Mcguire et al., Extragenic suppressors of the nimX2(cdc2) mutation of Aspergillus nidulansaffect nuclear division, septation and conidiation, GENETICS, 156(4), 2000, pp. 1573-1584
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,"Molecular Biology & Genetics
Journal title
GENETICS
ISSN journal
0016-6731 → ACNP
Volume
156
Issue
4
Year of publication
2000
Pages
1573 - 1584
Database
ISI
SICI code
0016-6731(200012)156:4<1573:ESOTNM>2.0.ZU;2-0
Abstract
The Aspergillus nidulans NIMXCDC2 protein kinase has been shown to be requi red for both the G(2)/M and G(1)/S transitions, and recent evidence has imp licated a role for NIMXCDC2 in septation and conidiation. While much is und erstood of its G(2)/M function, little is known about the functions of NIMX CDC2 during G(1)/S, septation, and conidiophore development. In an attempt to better understand how NIMXCDC2 is involved in these processes, we have i solated four extragenic suppressors of the A. nidulans nimX2(cdc2) temperat ure-sensitive mutation. Mutation of these suppressor genes, designated snxA -snxD for suppressor of nimX, affects nuclear division, septation, and coni diation. The cold-sensitive snxA1 mutation leads to arrest of nuclear divis ion during G(1) or early S. snxB1 causes hyperseptation in the hyphae and s ensitivity to hydroxyurea, while snxC1 causes septation in the conidiophore stalk and aberrant conidiophore structure. snxD1 leads to slight septation defects and hydroxyurea sensitivity. The additional phenotypes that result from the suppressor mutations provide genetic evidence that NIMXCDC2 affec ts septation and conidiation in addition to nuclear division, and cloning a nd biochemical analysis of these will allow a better understanding of the r ole of NIMXCDC2 in these processes.